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Natural Science Forum / Biology / Biology / March 2006help me how to grow yeast
Hi all, Well can simple media like PDA help me in growing yeasts? Or do I need some supplements? How do I prevent bacterial infections on my yeast? Please Help. > Well can simple media like PDA help me in growing yeasts? Or do I need > some supplements? Yeast can grow on a number of different media. The one I think most people use is called YPD: 20 g/l peptone 10 g/l yeast extract 2 % dextrose pH should be ~5.8 For plates, add agar before autoclaving. It is also a good idea to have a dextrose stock solution which has been sterilized by filtration and add the dextrose after autoclaving (and some cooling). Supplements may be needed if your yeast strain has special needs. BTW: Clontech has a nice manual available online (Yeast Protocols Handbook): http://www.clontech.com/clontech/techinfo/manuals/PDF/PT3024-1.pdf > How do I prevent bacterial infections on my yeast? Use sterile technique. cu Philipp  SignatureDr. Philipp Pagel Tel. +49-8161-71 2131 Dept. of Genome Oriented Bioinformatics Fax. +49-8161-71 2186 Technical University of Munich http://mips.gsf.de/staff/pagel |
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