how to test protein active?need help!

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知更鸟 - 18 Dec 2006 12:47 GMT

hello:
i'm a junior.my teacher told me to test the protein enzyme
active,how i can test it ?how about the LOWRY PROTEIN ASSAY?if you have
some articles about testing enzyme active or specific way of lowry
protein assay,can you send them to me?
i want to improve my specialty english ,if you have a good method of
improving it,can you write down on it.thank you!

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Bob - 19 Dec 2006 04:11 GMT

>hello:
> i'm a junior.my teacher told me to test the protein enzyme
[quoted text clipped - 3 lines]
>i want to improve my specialty english ,if you have a good method of
>improving it,can you write down on it.thank you!

Lowry is not relevant. It simply measures how much protein is present
by a chemical method; it does not matter if the enzyme is active or
not.

"Active" means that the protein is capable of doing what it is
supposed to do. You said that your protein is an enzyme. So you want
to test whether it works. That means knowing what it is supposed to
do, and then setting up an assay that is specific for this enzyme. If
it is a common enzyme, there probably are standard assays around. If
you have discovered a new enzyme, then it is for you to figure out how
to assay it -- and sometimes that can be a big job.

One part of assaying to see that your enzyme is active is to run a
control, in which you intentionally inactivate the enzyme. For most
enzymes from ordinary organisms, boiling them for a few minutes will
make them inactive.

If you google on the name of your enzyme plus the word assay, you will
probably find some assays. Also, if it is a common enzyme, try the web
site for Worthington Biochemicals. They also have a nice little
tutorial on enzymes.

bob

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Natural Science Forum / Biology / Biology / December 2006

how to test protein active?need help!