 |
 | Home | Contact Us | FAQ | Search & Site Map | Link to Us |
 | Sign In | Join | Other 45 Sites in Network |
Natural Science Forum / Biology / Biology / February 2007Looking for promoter sequences
Hello, I am trying to look for promoters of certain genes. I have tried using the NCBI websitee, but am not completely sure how to go about looking for them. I need some help in doing the same. Thanks Pooja On Feb 12, 10:07 pm, "poojamajmu...@gmail.com" <poojamajmu...@gmail.com> wrote: > Hello, > [quoted text clipped - 7 lines] > > Pooja hello. Pooja, try the following site where you will find links to several online promoter databases, and i hope you will find "promoters of certain genes" in one of the databases. the site is http://biophilessurf.info/ regards mahesh On Feb 12, 1:30 pm, "maheshe...@gmail.com" <maheshe...@gmail.com> wrote: > On Feb 12, 10:07 pm, "poojamajmu...@gmail.com" > [quoted text clipped - 8 lines] > > > Thanks > > Pooja > [quoted text clipped - 9 lines] > regards > mahesh Hi Mahesh, Thanks for your reply. I did go through the eukaryotic promoter database link that was put up on your website. I put the name of the proteins of interest, to look up their promoter sequences and I woul dnot get any answer. What should I do next?? Pooja >I did go through the eukaryotic promoter database link that was put up >on your website. I put the name of the proteins of interest, to look >up their promoter sequences and I woul dnot get any answer. > >What should I do next?? Mot sure what it is you are trying to do. The whole concept of a promoter for eukaryotic genes is something of a can of worms. You might want to look at a recent paper: Nature Methods - 3, 917 - 922 (2006) Rational design of a super core promoter that enhances gene expression Tamar Juven-Gershon, Susan Cheng & James T Kadonaga Might give you some ideas. Beyond that, how about expanding what it is you really want to know. bob > On 13 Feb 2007 06:51:55 -0800, "poojamajmu...@gmail.com" > [quoted text clipped - 19 lines] > > bob Hi Bob, Thanks for the input. what I am trying to look for are promoter sequences of certain Adhesion proteins such as PECAM-1, ICAM-1, E- Selectin .etc. which are expressed ont the surface of endothelial and certain other immune cells. I want to look if p53, the tumor suppressor gene consensus sequence is present on the promoters of these adhesion proteins. Pooja On Feb 14, 7:59 pm, "poojamajmu...@gmail.com" <poojamajmu...@gmail.com> wrote: > > On 13 Feb 2007 06:51:55 -0800, "poojamajmu...@gmail.com" > [quoted text clipped - 30 lines] > > Pooja hello, pooja, why did you try EPD only? you could try TRRD Cold Spring Harbor Laboratory Mammalian Promoter Database and there are many more. regards mahesh >> On 13 Feb 2007 06:51:55 -0800, "poojamajmu...@gmail.com" >> [quoted text clipped - 28 lines] >suppressor gene consensus sequence is present on the promoters of >these adhesion proteins. Ok, the point is to remember that "promoter" is not well defined for eukaryotic genes. Promoters are quite variable and diffuse; key elements of the promoter may be quite some distance away -- upstream or downstream. You need to look and see whether it has any particular type of promoter element, such as a TATA box or INR. You may want to look in some detail at papers on these genes, and see if there is info about what the key promoter elements are. In particular, do you understand the p53 promoter? bob |
Reply to this Message |
 Sign In
 Join
 My Latest Posts My Monitored Threads My Blog My Photo Gallery My Profile My Homepage Start New Thread Enable EMail Alerts Rate this Thread
|
©2009 Advenet LLC Privacy Policy - Terms of Use
This website includes both content owned or controlled by Advenet as well as content owned or controlled by third parties.