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Natural Science Forum / Biology / Biology / February 2007NMR and X-ray : relative qualities
Hi I am trying to learn about NMR and X-Ray to find out protein 3D structures. I have read from the web that the results from NMR and X- Ray could be quite different. But i do not have access to some papers that discuss about this. I read that NMR cannot handle large protein well but X-ray requires protein to be crystal form. I wish to get a feel from practitioners what are the relative qualities of each structure determined by these 2 methods ? presuming testing on a same protein. which results to believe ? Thank you Best Regards Boon Yiang >Hi > [quoted text clipped - 8 lines] >2 methods ? presuming testing on a same protein. which results to >believe ? It's not a matter of quality or "which to believe". They measure different things. X-ray analysis measures whatever you got into the crystal. NMR measures whatever you measured. Recent article -- Feb 8 issue of Nature -- on NMR with a large protein (670 KD): Quantitative dynamics and binding studies of the 20S proteasome by NMR pp618 - 622 By overcoming the molecular weight limitations that have traditionally hampered quantitative nuclear magnetic resonance spectroscopy (NMR) studies, the dynamics of the 670-kilodalton 20S proteasome core particle have been explored. A selective isotope labelling scheme along with experiments that preserve the lifetimes of the resulting NMR signals reveal functionally important motions and interactions by recording spectra on complexes with molecular weights up to 1 MDa. Remco Sprangers and Lewis E. Kay 10.1038/nature05512 Abstract: http://ealerts.nature.com/cgi-bin24/DM/y/ecQN0SpWd30HjB0BLkz0EH + News story Structural biology: Molecular machinery in action p609 Nuclear magnetic resonance is the best way to study motion in proteins, but it could be applied only to small systems. This limitation has been overcome to reveal the dynamics of a large protein complex. Ad Bax and Dennis A. Torchia 10.1038/nature05566 http://ealerts.nature.com/cgi-bin24/DM/y/ecQN0SpWd30HjB0BLkr0E8 Article descriptions above are from Nature TOC. bob |
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