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Natural Science Forum / Biology / Microbiology / June 2007aminoacids and bacterial growth
Is it possible to consider that some specific aminoacid are fundamentally essentials to (all) bacterial metabolism ? I am trying to interpret the growth of bacterial strains (lactic and non-lactic) with different peptones we have used in the lab, and I plan to perform aminoacid analysis to complete the nutrional pattern of the experimental culture media. Thank you for your help. Philip Gwerdan _________________________________________________________________ Voici le nouveau Windows Live Hotmail. Faites la mise à niveau gratuitement et voyez par vous-même. www.nouveauhotmail.ca?icid=WLHMFRCA123 >Is it possible to consider that some specific aminoacid are fundamentally >essentials to (all) bacterial metabolism ? I am trying to interpret the >growth of bacterial strains (lactic and non-lactic) with different peptones >we have used in the lab, and I plan to perform aminoacid analysis to >complete the nutrional pattern of the experimental culture media. You have a particular organism, and get varying responses to different peptones? You are wondering if you can account for that by the different amino acid compositions of the peptones? Maybe, but remember it is more complex. Peptones are usually not simply amino acids, but small peptides. These peptides compete for uptake systems. Then they compete for the peptidases. Then the amino acids affect regulatory mechanisms. So there are multiple levels at which the composition may affect the result. Dissecting these would be quite a challenge. What is you goal? Is it simply to improve growth, or do you intend this as a modeling study? If the goal is to understand the amino acid requirements of your organism, it would be much better to use defined mixes of amino acids. bob |
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