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Natural Science Forum / Biology / Microbiology / June 2008



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DAPI preparation

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mirzaman zamanzadeh - 28 Jun 2008 00:36 GMT
Hi,
 
I got a 5-mg DAPI powder from Fluka and I want to prepare a DAPI solution as follows:
 
 

Stock
Vol.
Work
Vol.

µg/mL
mL
µg/mL
mL

40
20
10
80
 
For taking powder, do I need to centrifuge the DAPI powder and then transfer it (5 mg) to a vial for making the stock solution.
 
Thanks in advance,
Mirzaman
N10 - 30 Jun 2008 22:42 GMT
Go ahead then

N10

Hi,

I got a 5-mg DAPI powder from Fluka and I want to prepare a DAPI solution as
follows:

Stock
Vol.
Work
Vol.

µg/mL
mL
µg/mL
mL

40
20
10
80

For taking powder, do I need to centrifuge the DAPI powder and then transfer
it (5 mg) to a vial for making the stock solution.

Thanks in advance,
Mirzaman
 
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