question about B. subtilis spore

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gh reza karbalaei - 20 Jul 2008 07:47 GMT

Hello
I research gram negative bacteria, but I need some information about B. subtilis that I cannot seem to find. I was wondering if anyone could answer some basic questions about this organism and possibly point me to some good review articles about the genetic manipulation and protocols for in in vitro growth.

Questions:

1) What is the composition of Difco sporulation media? I checked Difco and BD website but couldn't find this product. A google search also yielded no results. I could find no recipe.
2) Why does sporulation medium induce sporulation? Are there less nutrients present?
3) Does B. subtilis grow better at 30 C or 37 C?

Thanks for helping me out.

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JEDilworth - 21 Jul 2008 04:07 GMT

Bacillus subtilis is a gram positive rod last time I checked.

Judy Dilworth, M.T. (ASCP)
Microbiology

> I research gram negative bacteria, but I need some information about
> B. subtilis that I cannot seem to find.

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N10 - 22 Jul 2008 00:31 GMT

Greetings

1) What is the composition of Difco sporulation media? I checked Difco and
BD website but couldn't find this product. A google search also yielded no
results. I could find no recipe.

A bit more effort needed on your part on this one as the information is in
the Difco manual. These can be purchased on line.

2) Why does sporulation medium induce sporulation? Are there less nutrients
present?

Its a nutrient defficient medium which becomes rapdily depleted thus
inducing sporulation.

3) Does B. subtilis grow better at 30 C or 37 C?

Depends on the strain and medium used . Why not set up an experiment to
determine optimal growth for the strains you are working with.

Also Judy is correct B.subtilis is Gram positive.

Best N10

> Hello
> I research gram negative bacteria, but I need some information about B.
[quoted text clipped - 13 lines]
>
> Thanks for helping me out.

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Bob - 22 Jul 2008 04:17 GMT

>Hello
>I research gram negative bacteria, but I need some information about B. subtilis that I cannot seem to find. I was wondering if anyone could answer some basic questions about this organism and possibly point me to some good review articles about the genetic manipulation and protocols for in in vitro growth.
>
>Questions:

I emailed you a reply with info on DSM. (Don't know if you check your
email regularly.)

bob

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Natural Science Forum / Biology / Microbiology / July 2008

question about B. subtilis spore