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Natural Science Forum / Biology / Microbiology / July 2007Actinomyces pyogenes
Hi, Can anyone tell me some biochemical test of Actinomyces pyogenes?? Or if A. pyogenes is PYR negative?? Cant find nothing about it..... and i have to differenciate A. pyogenes from Streptococcus pyogenes....!!! If anyone knows anything about Actinomyces pyogenes, tell me something.... I would appreciate very much!! Thank You > Hi, > Can anyone tell me some biochemical test of Actinomyces pyogenes?? Or [quoted text clipped - 4 lines] > something.... I would appreciate very much!! > Thank You Come on!! Even a simple Google search produced 35,300 hits! And if you cannot differentiate between actinomyces and strep, you haven't learned anything in your microbiology class. Do not expect us to do your homework for you.  SignatureLarry D. Farrell, Ph.D. Professor of Microbiology Idaho State University -- Posted via a free Usenet account from http://www.teranews.com If it is that easy, does anybody knows if Actinomyces pyogenes is PYR ( L-pyroglutamic acid beta-naphthylamide ) positive ??? Thats the only thing i'm asking!!! And of course i haved search in google.... and in other places and in books.... and i cant find it.... Thanks anyway!!! > If it is that easy, does anybody knows if Actinomyces pyogenes is PYR > ( L-pyroglutamic acid beta-naphthylamide ) positive ??? Thats the only > thing i'm asking!!! And of course i haved search in google.... and in > other places and in books.... and i cant find it.... Thanks anyway!!! If that is all you needed to know, why didn't you ask that at the outset instead of asking if "anyone knows anything about A. pyogenes?" SignatureLarry D. Farrell, Ph.D. Professor of Microbiology Idaho State University -- Posted via a free Usenet account from http://www.teranews.com It's probably not a test that is used to identify A. pyogenes, which is why you can't find it. We use PYR mostly to identify enterococci. It CAN be used for Strep. pyogenes, but only as an ancillary test if you need it. I think it can be used to differentiate a few gram negatives also. Judy Dilworth, M.T. (ASCP) Microbiology > If it is that easy, does anybody knows if Actinomyces pyogenes is PYR > ( L-pyroglutamic acid beta-naphthylamide ) positive ??? You're right Judy. The identification should have started with a Gram stain - the Gram pos rods bring the organim id down a different path. SignatureJohn Gentile MS, M(ASCP) Laboratory Information Mgr. VA Medical Center Providence, RI yjgent@cox.net > It's probably not a test that is used to identify A. pyogenes, which is > why you can't find it. [quoted text clipped - 8 lines] >> If it is that easy, does anybody knows if Actinomyces pyogenes is PYR >> ( L-pyroglutamic acid beta-naphthylamide ) positive ??? We've all been down the "wrong path" because we didn't make a simple gram stain. It is extremely important to know what morphology you are dealing with before you proceed. I've been burned in the past so I make this a necessary step. I gram smear any coagulase negative "staphylococci" to make SURE they are indeed cocci. Every once in awhile I end up with diphtheroids. Alpha "strep" can fool you and turn out to be Lactobacilli (long gram positive rods). I've seen "mycobacteria" on a Lowenstein Jensen slant turn out to be yeast because the tech just assumed that anything that grew on an LJ was automatically acid fast and sent it out for identification. Our Ph.D. just shook his head at that one. It makes the sender look pretty dumb. So, to all you students and newbie micro techs out there: when in doubt, stain your colony to find out what you have before you proceed. You'll be glad you did. Judy Dilworth, M.T. (ASCP) Microbiology > You're right Judy. The identification should have started with a Gram > stain - the Gram pos rods bring the organism id down a different path. Actinomyces are gram positive rods. Streptococci are gram positive cocci in chains. S, pyogenes is beta hemolytic on 5% TSA sheep blood agar. S. pyogenes, known otherwise as Group A beta streptococcus, is PYR positive. Have you performed a gram stain? What medium are you using to grow the organisms? Strep can sometimes look a bit elongated on gram smear, but you should still see some cocci in chains on your smear. Remember: in microbiology PERFORM A GRAM STAIN when in doubt. It will clear up LOTS of questions. I just googled A. pyogenes and got 134,000 (!) hits. Have you thought of using the internet? A. pyogenes is now known as Arcanobacterium pyogenes, which I have heard of. Some labs identify these in strep screen throat cultures; others do not. It has been implicated in throat infections. http://www.google.com/search?hl=en&q=Actinomyces+pyogenes (my google search). Judy Dilworth, M.T. (ASCP) Microbiology > Hi, > Can anyone tell me some biochemical test of Actinomyces pyogenes?? Or [quoted text clipped - 4 lines] > something.... I would appreciate very much!! > Thank You I've run into this organism a couple of times, and the most interesting case was a positive blood culture. I used a Gram positive rod approach to the identification, but the hemolysis was not very apparent on the horse blood agar we were using at the time. After a couple of days we finally did ID it and also checked the patient's previous cultures. I found that he had been admitted with a leg wound and a culture of the wound was reported out as Diphtheroids. I found the original plates and realized that the tech had missed the hemolysis and didn't go any further. It made an interesting case for our microbiology rounds. > Actinomyces are gram positive rods. Streptococci are gram positive > cocci in chains. S, pyogenes is beta hemolytic on 5% TSA sheep blood [quoted text clipped - 19 lines] > Judy Dilworth, M.T. (ASCP) > Microbiology SignatureJohn Gentile MS, M(ASCP) Laboratory Information Mgr. VA Medical Center Providence, RI yjgent@cox.net |
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