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Natural Science Forum / Biology / Microbiology / July 2007



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What is reversed passive hemagglutination assay method and how it is performed

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Lincoln - 03 Jul 2007 23:48 GMT
Hi all,

I tried to search on several search engines and medical university web
sites
yet had not found the answer.

1. What is the reversed passive hemagglutination assay method

2. Why the "reversed passive"? What does it signify?

3. How to perform this?

Thank you,

Lincoln

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Larry Farrell - 04 Jul 2007 05:21 GMT
Google  returned 795 hits, the first of which was a definition.  You
need to use better search engines.

> Hi all,
>
[quoted text clipped - 13 lines]
>
> --Using Opera's revolutionary e-mail client: http://www.opera.com/mail/

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Larry D. Farrell, Ph.D.
Professor of Microbiology
Idaho State University

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Lincoln - 04 Jul 2007 10:44 GMT
Thanks for the response. I have used Google among several search engines,  
and the first result is not a definition. It is merely acronym. The other  
results talk about things like "Cryptosporidium antigen detection in human  
feces by reverse passive hemagglutination assay."

Pardon me, I should have elaborated a bit more. I know what is  
hemagglutination assay. However, I cannot find the meaning for "reverse  
passive". I was listening to the professor's lecture but he couldn't  
explain (note, English is not his first language) well the difference  
between "reverse passive hemagglutination assay" and "hemagglutination  
assay".

So if anyone can enlighten me about the "reserve passive" part, I would  
really appreciate it.

Lincoln

> Google  returned 795 hits, the first of which was a definition.  You  
> need to use better search engines.
[quoted text clipped - 9 lines]
>>  Lincoln
>>  --Using Opera's revolutionary e-mail client: http://www.opera.com/mail/

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Kay Lancaster - 04 Jul 2007 22:42 GMT
I've got to agree with Larry... you need a better search engine or a better
search strategy.

Try this google search:

>>>  "reverse passive agglutination" glossary  <<<

(leave out the angle brackets")

Now look for "reverse passive agglutination" within the first of the two hits.
Then read the other definitions in the agglutination section.

Your reference librarian should be able to help you learn to use a search
engine more effectively.
Lincoln - 05 Jul 2007 01:46 GMT
Thank you for your help. With your specific recommendation (-assay,  
+glossary), I found what I was looking for. Unfortunately, the university  
does not a reference librarian. I am rather good with computers and  
programming, but this is a bit domain-specific to microbiology. I'm glad  
you were able to point me to the right direction.

From: Pure & Appl. Chern., Vol. 66, No. 12, pp. 2587-2604, 1994.

Hemagglutination - Agglutination reactions in which the particles used are  
erythrocytes.
Hemagglutination may be either dircct, in which erythrocyte antigens are  
reactants, or
indirect (passive) for coated antigcn or, in the case of reverse (passive)  
assays, coated
antibody. One of the most common uses of hemagglutination is to quantitate  
the number
of hemagglutinating viruses (cl: influcnza) or their soluble  
hemagglutinating surface
subunits (ref. 22).

Indirect (Passive) Agglutination - The agglutination technique in which  
antigen first is
coated artificially onto the particulate surfaces, either by physical  
absorption or chemical
or immunochemical linkage. Thcse antigen-laden particles then can be used  
to detect the
presence of the corresponding spccific agglutinins in test material.  
Agglutination results
by cross-linking of the antigen-bearing particles onto an extensive  
antigen-antibody lattice
(i.e., in detectable agglutination of the particlcs) (rcf. 22).

I hope this will stay in the usenet archive since Google didn't return so  
many results.

Lincoln

> I've got to agree with Larry... you need a better search engine or a  
> better
[quoted text clipped - 12 lines]
> Your reference librarian should be able to help you learn to use a search
> engine more effectively.

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