PCR directly on stool specimens

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Morgan - 24 Jan 2005 20:05 GMT

I am wondering if any of you have protocols about PCR techniques and
bacterial DNA extractions directly from healthy swine stools specimens.

Thank you

Morgan

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Scott Coutts - 25 Jan 2005 02:58 GMT

> I am wondering if any of you have protocols about PCR techniques and
> bacterial DNA extractions directly from healthy swine stools specimens.

You'd be surprised what even regular google will bring up for you. I bet
if you do a search for "swine stool pcr" you'll come up with something
useful. Otherwise, use the same search terms to check the literature and
you should come up with something

http://www.ncbi.nlm.nih.gov/gquery/gquery.fcgi

Cheers,

Scott.

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Ecoli - 26 Jan 2005 18:11 GMT

The first thing that came in mind is DGGE (denaturing gradient gel
electrophoresis)
(or TRFLP - terminal restriction fragment length polymorphism)

use this terms in combination with stool, faeces ... and u will certainly
find something

> > I am wondering if any of you have protocols about PCR techniques and
> > bacterial DNA extractions directly from healthy swine stools specimens.
[quoted text clipped - 9 lines]
>
> Scott.

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Natural Science Forum / Biology / Microbiology / January 2005

PCR directly on stool specimens