 |
 | Home | Contact Us | FAQ | Search & Site Map | Link to Us |
 | Sign In | Join | Other 45 Sites in Network |
Natural Science Forum / Biology / Microbiology / June 2005Help - BspGI restriction enzyme!
Can anyone tell me where to get it? It's not commercially available > Can anyone tell me where to get it? It's not commercially available most of the time it's worthwhile to find a way around unavailable RE try using PCR mutagenesis to give yourself a useful site this will only take you perhaps a few extra primers and about two days total time the savings in time will far outweigh looking for a rare/unpopular RE > Can anyone tell me where to get it? It's not commercially available Why do you need that specific enzyme? Surely the presence of a CCCGT site can't be that big an issue in your application. What about using Fau I, which has a CCCGC site?  SignatureLarry D. Farrell, Ph.D. Professor of Microbiology Idaho State University I need this enzyme to genotype a polymorphism by RFLP. None of the more modern methods work for my SNP, which is: CTGGA[A/G]. This is the only enzyme that could differentially cut each allele. > I need this enzyme to genotype a polymorphism by RFLP. None of the more > modern methods work for my SNP, which is: CTGGA[A/G]. This is the only > enzyme that could differentially cut each allele. Since Bpm I cuts CTGGAG, I don't see why it could not be used. SignatureLarry D. Farrell, Ph.D. Professor of Microbiology Idaho State University The big question now, since there has been no reply by Maya, is whether any of this helped at all? I, for one, would certainly like to know. >> I need this enzyme to genotype a polymorphism by RFLP. None of the more >> modern methods work for my SNP, which is: CTGGA[A/G]. This is the only >> enzyme that could differentially cut each allele. > > Since Bpm I cuts CTGGAG, I don't see why it could not be used. Sorry for the delay! I had tried most of the things you suggested before putting the question online as the NEB program I used to find the enzyme in the first place has a direct link to REBASE. I haven't tried writing to NEB directly for advice. The other enzyme suggested doesn't work - I think I made a mistake in the sequence I put up here, sorry! Dr. Mayr's contact doesn't seem to be working either - I tried faxing and it came back. The best solution I could come up with was to try and genotype another SNP with which this one I wanted seems to be in complete linkage desequilibrium in some populations. Not ideal, but the best I could come up with. I put this question up more in the hope that someone had needed this enzyme before and had managed to obtain it. But any further suggestions are very welcome and thanks for all the suggestions given! >I need this enzyme to genotype a polymorphism by RFLP. None of the more >modern methods work for my SNP, which is: CTGGA[A/G]. This is the only >enzyme that could differentially cut each allele. If Larry hasn't solved your problem, why not contact the folks at New England Biolabs for advice. bob >>I need this enzyme to genotype a polymorphism by RFLP. None of the more >>modern methods work for my SNP, which is: CTGGA[A/G]. This is the only >>enzyme that could differentially cut each allele. > > If Larry hasn't solved your problem, why not contact the folks at New > England Biolabs for advice. Otherwise, check this resource, which is very useful http://rebase.neb.com/rebase/rebase.html >>>I need this enzyme to genotype a polymorphism by RFLP. None of the more >>>modern methods work for my SNP, which is: CTGGA[A/G]. This is the only [quoted text clipped - 6 lines] > >http://rebase.neb.com/rebase/rebase.html I'm sure Scott knows this, but that rebase web site is from the company I mentioned. bob >>>>I need this enzyme to genotype a polymorphism by RFLP. None of the more >>>>modern methods work for my SNP, which is: CTGGA[A/G]. This is the only [quoted text clipped - 9 lines] > I'm sure Scott knows this, but that rebase web site is from the > company I mentioned. Yep, but I mentioned rebase in particular, rather than just NEB, because if you look at the NEB website and view their catalog, you get only NEB enzymes... if you look at rebase, you get all enzymes, even commercially unavailable ones (: Cheers, Scott. |
Reply to this Message |
 Sign In
 Join
 My Latest Posts My Monitored Threads My Blog My Photo Gallery My Profile My Homepage Start New Thread Enable EMail Alerts Rate this Thread
|
©2009 Advenet LLC Privacy Policy - Terms of Use
This website includes both content owned or controlled by Advenet as well as content owned or controlled by third parties.