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Natural Science Forum / Biology / Microbiology / February 2006



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Motile fungi

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Gary G - 12 Feb 2006 03:29 GMT
Are thre motle fungi?  I thought that motile individuals were
moving via flagella and were bacteria.  What I am dealing with now is

"Google zoospores and you will find that contrary to all other fungus
they are mobile with both amoebic motion and flagella. The info came
from more than one source, zoospores are just not generally well
known."

Is this true?

The fungi in question are at least 75 million years old.

Gary Gaugler, Ph.D.
Microtechnics, Inc.
Granite Bay, CA 95746
916.791.8191
gary@microtechnics dot com
Bob - 13 Feb 2006 05:08 GMT
>Are thre motle fungi?  I thought that motile individuals were
>moving via flagella and were bacteria.  What I am dealing with now is
[quoted text clipped - 5 lines]
>
>Is this true?

yes

The oomycetes are aquatic fungi (unusual) and have motile spores
(unusual, but perhaps appropriate). It is often said that they do not
fit well in the fungi group. Certainly, they are talked about much
less than common molds, yeasts, mushrooms.

I wonder if the new proposal to reclassify eukaryotic microbes does
anything new with them.

bob
Gary G - 13 Feb 2006 17:42 GMT
>>Are thre motle fungi?  I thought that motile individuals were
>>moving via flagella and were bacteria.  What I am dealing with now is
[quoted text clipped - 17 lines]
>
>bob

Thanks for the reply.  These apparent fungi are all that remain in the
vessels of femur from 75M year old dinosaur findings.  The species are
believed to be T rex or Triceratops.  Here are some pix of the fungi
clusters:

http://www.microtechnics.com/ball2area-1kx-1.jpg

http://www.microtechnics.com/femur-2-2200x-1.jpg

The apparent soft tissue in the bone or the bone itself shows tracks
where some organism (the fungi?) had moved all over and displaced and
consumed(?) some of the material.

If you or anyone else has any idea, please let me know.  all input is
appreciated.

Gary Gaugler, Ph.D.
Microtechnics, Inc.
Granite Bay, CA 95746
916.791.8191
gary@microtechnics dot com
rene - 15 Feb 2006 19:21 GMT
We had plenty of infectious fungal parasites in our marine diatom
cultures. They are very common flagellates that settle on a substrate,
shed their flagella and become a zoospore. No doubt that will happen
with your stuff if it sinks to the bottom of the sea before
sedimentation.
As said, their relation with the true fungi is doubtful but they are
certainly eukaryotic.

Nice images, btw!

René
Gary G - 16 Feb 2006 23:25 GMT
>We had plenty of infectious fungal parasites in our marine diatom
>cultures. They are very common flagellates that settle on a substrate,
[quoted text clipped - 7 lines]
>
>René

Thanks very much for the info and insite and for the feedback on
images.  This all helps a lot.

These specimens are probably from fresh water areas that were inland
lakes or even part of the sea (than salt-based) millions of years ago.
EDS shows trace amounts of Na and Cl.  So it might indicate that the
speicmens are from an inland sea.

In fossiled state, the zoospores are primarily composed of
O, Fe and P according to EDS analysis.  The surrounding area is mostly
Si, Al and Fe.  Others have reported C, Ti, O, Si, P, Cl and K.  When
the fossilized femur is converted to soft tissue and minerals are
removed using HF, tracks are seen in the assumed tissue.  Do you think
that these tracks are consistent with what a zoospore would do?

See image at:

http://www.microtechnics.com/bridge1.jpg

I still have to sort out the elemental complement based on some trace
elements.

Thanks.

Gary Gaugler, Ph.D.
Microtechnics, Inc.
Granite Bay, CA 95746
916.791.8191
gary@microtechnics dot com
jclausz@cc.edu - 17 Feb 2006 05:26 GMT
Gary,

In the kingdom Fungi therre are 4 divisions or phyla. They all are
characterized by having vegetative structures composed of chitinous
cell wall, unlike plants which have primary walls made of cellulose.
One of the 4 phyla - the chytridiomycota also called chytrids - have
flagella. The other 3 phhyla - Zygomycota, Ascomycota and Basidiomycota
- do not produce cells with flagella on them. The chytrids produce
cells with a single, posteriorally-oriented, whiplash type flagellum.
To see what these flagellated cells look like, do a Google image search
with the name Allomyces.

Another responder wrote to you about the group of "aquatic fungi"
called the Oomycetes. These organisms are no longer thought to be true
fungi because they produce cell walls of cellulose. They are more
animal-like than they are fungal-like based on DNA sequence analysis.
These water molds do have flagellated cells but there are two flagella
on each motile cell. One is a posteriorly-oriented, whiplash type
flagellum and the other is anteriorly-orented, tinsel type flagellum.
To see one of these zoospores do a Google image search on the name
Saprolegnia.

I hope this helps.

John Clausz

> Are thre motle fungi?  I thought that motile individuals were
> moving via flagella and were bacteria.  What I am dealing with now is
[quoted text clipped - 13 lines]
> 916.791.8191
> gary@microtechnics dot com
Gary G - 18 Feb 2006 00:42 GMT
>Gary,
>
[quoted text clipped - 21 lines]
>
>John Clausz

Thanks for the response.

All input helps.  The problem I face is trying to correlate what I see
today for specimens that are millions of years old.  I am not used to
this.  Thus, the dilemma is to sort out what is valid today relative
to what is seen in very old specimens.  Is/are the principles of what
we know today applicable to legacy specimens?  In this case, really
old specimens.

Converting fossilized specimens into soft tissue involves a lot of
work.  What I fear is that this process will distort the true nature
of the specimen.  Demineralization is a big deal.  If I use CaCl2 or
GuHCl or MgCl2, et. al., then what does this do to the specimen and
actual EDS results?  My other method is buffered HF (HF+Ammonium
Flouride).  Tricky.

Gary Gaugler, Ph.D.
Microtechnics, Inc.
Granite Bay, CA 95746
916.791.8191
gary@microtechnics dot com
 
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