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Natural Science Forum / Biology / Microbiology / January 2007Problems with Pseudomonas Isolation Agar
Hi all, I've a problem with Pseudomonas aeruginosa PAO1. Briefly, it doesn't grow on Luria-Bertani medium plates added with Tetracycline (30 microg/ml) but it grows if the same amount of tetracycline is added to Pseudomonas Isolation Agar (Difco). Pseudomonas putida KT2440 doesn't grown on the same medium, so this means that there isn't a problem with Tetracycline. PAO1 grows also in PIA+Tetracycline without glycerol (that is a component of PIA). I really don't understand what's happening!!!!! Someone could help me? Thanks Andrea dear andrea as per my knowledge (hi media in india) luria bertani agar is used for isolation of E .coli ATCC 25922 and 23724 it is specially designes by lennox for cultivation and maintainence of recombinanat strains of E coli. further iam searching for ur pseudomonas problem. regards > Hi all, > I've a problem with Pseudomonas aeruginosa PAO1. Briefly, it doesn't [quoted text clipped - 7 lines] > Thanks > Andrea >Hi all, >I've a problem with Pseudomonas aeruginosa PAO1. Briefly, it doesn't [quoted text clipped - 5 lines] >(that is a component of PIA). I really don't understand what's >happening!!!!! What is happening that shouldn't? It is hard to tell from what you wrote above. It is certainly normal enough that the allowable level of a drug depends on the base medium it is added to. bob |
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