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Natural Science Forum / Biology / Microbiology / February 2007B. megaterium pigment
Hi all, I recently obtained a culture of Bacillus megaterium, and as it sporulated, it secreted a dark gray or black pigment. Does anyone have a reference they could suggest on this? Thanks! Chris Hey chris, i'm vanessa from Portugal....did you already try to see in "microbiology of Brock", it's a good reference for microbiology....or "atlas of microbiology".... but if u want to tell me what is your objective for your study os Baccilus megaterium, i could try to see more os it.... i'm studing microbiology, and i am almost finish it. > Hi all, > [quoted text clipped - 3 lines] > > Chris >Hi all, > >I recently obtained a culture of Bacillus megaterium, and as it >sporulated, it secreted a dark gray or black pigment. Does anyone have >a reference they could suggest on this? Thanks! Hm, some bacilli make melanin. Is that plausible here? Melanin production often responds to level of tyrosine in the medium. bob > On 14 Feb 2007 09:27:32 -0800, "chris.linthomp...@gmail.com" > [quoted text clipped - 9 lines] > > bob Hi folks...I found a really weird thing here. There's a yeast that lives symbiotically with B. megaterium, and that's what is discoloring the agar. Neat, but bizarre. I will try to dig up a reference. Thanks Vanessa & Bob! Chris On Feb 22, 4:42 pm, "chris.linthomp...@gmail.com" <chris.linthomp...@gmail.com> wrote: > > On 14 Feb 2007 09:27:32 -0800, "chris.linthomp...@gmail.com" > [quoted text clipped - 17 lines] > > Chris Chris - you're inability to establish a pure culture doesn't mean you can claim higher level assocations such as symbiosis. go learn aseptic technique, On Feb 23, 12:53 am, lobo.jorge.l...@gmail.com wrote: > On Feb 22, 4:42 pm, "chris.linthomp...@gmail.com" > [quoted text clipped - 26 lines] > > go learn aseptic technique, Heh. I didn't make the claim. I was told about this by a microbiologist of over 30 years experience, who's done it all- clinical stuff for the army, academics (doctorate from Rutgers), ran his own commercial lab with a dozen employees. If I had found it, I WOULD have assumed contamination. Seems like I am not the one making assumptions here. Chris >> > Hi folks...I found a really weird thing here. There's a yeast that >> > lives symbiotically with B. megaterium, and that's what is discoloring >> > the agar. Neat, but bizarre. Ah, Meg has a pet. So cute. :-) >> > I will try to dig up a reference. Thanks Vanessa & Bob! >> >> > Chris ... >Heh. I didn't make the claim. I was told about this by a >microbiologist of over 30 years experience, who's done it all- >clinical stuff for the army, academics (doctorate from Rutgers), ran >his own commercial lab with a dozen employees. If I had found it, I >WOULD have assumed contamination. There is a separate post, titled Bacillus megaterium, on yeasts making melanin. Is that supposed to be related here? There is nothing in the part that is posted that makes any connection to B meg. That some fungi can make melanin is beside the point; so can Bacillus sp. (And so can people.) I did some brief searching, and found nothing to indicate that any such connection is common. I did not try to be exhaustive. So, I'm open, but skeptical. In particular, I am skeptical that there is any "usual" association. That certainly doesn't preclude that you have something (or that I am wrong). Why do you think you have a bacillus-yeast system? And what are you saying about it? Why do you call it a symbiosis? Are the cells associated physically, or is it a mixed culture? If the latter, what does it look like under microscope, and can you "cure" it of one partner with the expected drugs, or with single colony isolation? bob > On 23 Feb 2007 03:41:32 -0800, "chris.linthomp...@gmail.com" > [quoted text clipped - 37 lines] > > bob OK Bob Iam sorry but I cant change the title of my post now.If I can please tell me HOW? THANKS.. >OK Bob Iam sorry but I cant change the title of my post now.If I can >please tell me HOW? >THANKS.. If it was intended as a reply to a posted message, then it probably should have been posted by using the reply feature -- as you did here. That is how message threads are made. But more important is the science here. I think most important is info from Chris about what he actually observed. But as to your post and paper: why do you think that has anything to do with B meg? (Obviously, the paper is of some interest for the reasons stated in the abstract. But it is not obvious that it has anything to do with the original question. I'll look up the paper later, just for curiosity.) bob > >OK Bob Iam sorry but I cant change the title of my post now.If I can > >please tell me HOW? [quoted text clipped - 13 lines] > > bob I well answer your question.. actually i want to make reply to this subject and i dont know HOW(i meen it) then i make a post. when i looked to the subject space..i wrote B. megaterium to make reply for this text. (its an error) :-) > >OK Bob Iam sorry but I cant change the title of my post now.If I can > >please tell me HOW? [quoted text clipped - 13 lines] > > bob I also am trying to track down some references. I have spent a couple days searching and there is almost nothing coming up anywhere, which is odd to say the least. What I got from my colleague is the name _Hansula_, but there doesn't seem to be any yeast by that name- except one or two mispellings of _Hansenula_. The description of the little bugger is that it looks like a "derby hat"- meaning a slightly flattened cell, almost an oval, with some sort of little protrusions on one end that make it look like the rim of a hat. I did see something like that on stains I've made. I would be surprised if this guy was mistaken, since as I said he has a real wealth of experience. But what the heck, everyone misses on Jeopardy once in a while, right? Thanks for the help, all. It's an interesting little problem, and all the points of view are valuable. The last reason I don't think it's a contaminant- or at least one that I introduced, rather than one that came with the culture- is that all my other stocks (I keep about 6 things growing at any one time during the semester) are clean, even the ones on the same batch of tryptic soy slants the megaterium is growing on. Chris >The last reason I don't think it's a contaminant- or at least one that >I introduced, rather than one that came with the culture- is that all >my other stocks (I keep about 6 things growing at any one time during >the semester) are clean, even the ones on the same batch of tryptic >soy slants the megaterium is growing on. If I understand your point, it does not distinguish the pigment being from the yeast or from meg itself. The key test might well be single colony isolation, coupled with some microscopic observation. Let us know! bob > On 26 Feb 2007 06:32:14 -0800, "chris.linthomp...@gmail.com" > [quoted text clipped - 15 lines] > > bob Will do! Chris |
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