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Natural Science Forum / Physics / Optics / November 2006Zeiss phase condenser
Can anyone helop with advice about this condenser, please? It is CZ with the serial number 4337802. there is an open position, marked J, a darkfield stop , marked D, and 4 phase rings, marked 2, 3,2,3. These differ in that two are translucent, and two are reflective. Can anyone tell me what these different types of phase stop are for, please? I assume that one pair is for normal phase, but the other? Thanks for any information/advice/experience. I haven't got any phase objectives to try it with yet, so am anxious to get suitable ones for this condenser! I think what you have there is a set of 2 positive phase plates (the translucent ones) and a set of 2 negative (the opaque ones). Look in http://www.microscopyu.com/articles/phasecontrast/phasemicroscopy.html for more info... Cheers Beats > Can anyone helop with advice about this condenser, please? It is CZ > with the serial number 4337802. [quoted text clipped - 6 lines] > objectives to try it with yet, so am anxious to get suitable ones for > this condenser! > I think what you have there is a set of 2 positive phase plates (the > translucent ones) and a set of 2 negative (the opaque ones). Look in [quoted text clipped - 15 lines] > > objectives to try it with yet, so am anxious to get suitable ones for > > this condenser! Many thanks to all for very helpful advice, hugo j Do you actually see a ring in the translucent ones?? > > I think what you have there is a set of 2 positive phase plates (the > > translucent ones) and a set of 2 negative (the opaque ones). Look in [quoted text clipped - 17 lines] > > Many thanks to all for very helpful advice, hugo j Yes, the ring is visible > Do you actually see a ring in the translucent ones?? > [quoted text clipped - 19 lines] > > > > Many thanks to all for very helpful advice, hugo j Never heard of before. So if you're looking in the scope without eyepiece but with the phase stops in the condenser, what do you see? Oncologists schreef: > Yes, the ring is visible > > Do you actually see a ring in the translucent ones?? [quoted text clipped - 20 lines] > > > > > > Many thanks to all for very helpful advice, hugo j > Never heard of before. So if you're looking in the scope without > eyepiece but with the phase stops in the condenser, what do you see? 1 semi-opaque background with clear ring, 1 completely opaque background with clear ring > Oncologists schreef: > [quoted text clipped - 22 lines] > > > > > > > > Many thanks to all for very helpful advice, hugo j Amazing, never seen before. I know of phase condensers for fluorescence which let short wavelength light (UV) through, but not this. This sounds like it would 'dilute' the phase contrast effect, which is quite handy as the phase contrast often is too strong. Just a thought: try a polarizer under the condenser. It *might* give a variable blocking of light, although I would expect clear (non-diffused) light going through the condenser instead of translucency/opacity. As far as I am aware, negative and positive phase contrast is done in the objective, not by the condenser. Ren?. Oncologists schreef: > > Never heard of before. So if you're looking in the scope without > > eyepiece but with the phase stops in the condenser, what do you see? [quoted text clipped - 27 lines] > > > > > > > > > > Many thanks to all for very helpful advice, hugo j The number on the phase objective needs to match the number on the phase turret. This is an interesting article about phase microscopes: http://www.microscopy-uk.org.uk/mag/artaug02/pjphase.html > Can anyone helop with advice about this condenser, please? It is CZ > with the serial number 4337802. [quoted text clipped - 6 lines] > objectives to try it with yet, so am anxious to get suitable ones for > this condenser! Some countries use the comma for a decimal point so 3,2 would be 3.2. > Can anyone helop with advice about this condenser, please? It is CZ > with the serial number 4337802. [quoted text clipped - 6 lines] > objectives to try it with yet, so am anxious to get suitable ones for > this condenser! |
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