I have a microscope which came with a blue and a green filter. I use the blue to
create a white background. I can find anyone that knows what you would use the
green filter for. Any help?
Rainer Teubner - 28 Mar 2007 10:28 GMT
Hello,
the green filter is for illumination for phase-contrast according to
Zernike. As the human eye has its maximum sensibility in the green part
of the optical spectrum, chromatic errors of the achromatic objectives
are minimised ba the green filter.
Kind regards
Rainer Teubner
sathered@wvi.com schrieb:
> I have a microscope which came with a blue and a green filter. I use the blue to
> create a white background. I can find anyone that knows what you would use the
> green filter for. Any help?
Trond Kvitvik - 28 Mar 2007 19:59 GMT
> Hello,
>
[quoted text clipped - 13 lines]
>> use the
>> green filter for. Any help?
The green filter is used for B&W (film) photography, especially when
using achromatic objectives. Black and white photographic film has a
different sensitivity for the colour spectrum than the human eye (and no
'correction in the brain'). The chromatic aberrations of an objective
will be more visible in an B&W photo, than what appears when we look
through the oculars.
A green filter is used to reduce the effects of chromatic aberration.
PS: I'm not sure of the differences in practice between using
Pan-chromatic or Ortho-chromatic B&W film, the latter is not sensitive
to red light.

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Trond Kvitvik (M.Sc Botany)
Fin-00170 Helsinki
Address & Tel: "http://home.no.net/tkvitvik/images/address.gif"
heini - 28 Mar 2007 12:41 GMT
Hi,
you are not creating "white light" with a blue filter!
As lenses are something like round prisms, light containing all
wavelength from red to blue would be split up each time light is
reflected or bent, resulting in chromatical aberrations.
So it makes sense to take one part only, the red or green or blue
section.
As resolution is depending on the wavelength, is makes sense to take
short wavelength light: blue
That's what the blue filter is for!
On Mar 28, 7:37 am, sathe...@wvi.com wrote:
> I have a microscope which came with a blue and a green filter. I use the blue to
> create a white background. I can find anyone that knows what you would use the
> green filter for. Any help?
yours,
Heinrich
Gary G - 29 Mar 2007 01:24 GMT
>Hi,
>you are not creating "white light" with a blue filter!
[quoted text clipped - 14 lines]
>yours,
>Heinrich
The "white background" is the topic.
It is a color temperature issue. There are blue filters that are
indeed blue. I suspect the one you have is a daylight correction
filter. This converts the 2900K halogen to 5500K daylight for
daylight color film. Of course it isn't exact since much depends on
the intensity of the halogen light source. But assuming it is around
2900K, then the conversion is good.
The green filter has several uses but I think the main one is for high
contrast with b/w film. It can also be used with monochrome CCD
cameras.
gg
Kiss French. Drink California.
gary at gaugler dot com
Kevin Cunningham - 29 Mar 2007 12:42 GMT
>>Hi,
>>you are not creating "white light" with a blue filter!
[quoted text clipped - 35 lines]
>
> gary at gaugler dot com
Gary, One point is that there are soooo many "blue" and "green" filters.
Now mostly green filters are used for things like phase contrast or for
making a better looking specimen by restricting the range wave lengths of
incoming light to the objective. If you restrict the wavelengths you get an
apochromatic effect with achromat objectives as long as the specimen is
naturally black and white like chromosomes. Now with interference filters
its real easy to get exactly the blue or green the customer wants.
When I started my company I thought I'd always keep a blue filter in my tool
box and when a customer needed one I'd throw the new one on the microscope,
thus ends the discussion. Jeez was I ever wrong. Either it was to blue or
not blue enough, to thick or not thick enough. I hope I finally threw it
out of my speeding car! There are a bunch of filters to try if you want to
fool around with the instrument, blues, greens and (my fave) didymium.
Thanks,
Kevin Cunningham
SMS
gto - 30 Mar 2007 08:12 GMT
What type of green filter is it?
If it is a color contrast filter, you can use it to enhance contrast for
certain specimen. You most like use then a B&W image sensor.
If it is an interference filter, you can use it for phase contrast
illumination to improve the phase contrast effect.
Cheers,
Gregor
PS: The following is from one of my old web pages:
Color Contrast Filters
These filters are chosen to be complementary in color to whatever color in
the object is to appear dark on B&W film. For instance, take a red R(25A)
filter if you want the blue sky to appear black. Mostly, these filters are
based on selective absorption and transmission. The glass filters from HOYA
type red R(25A), green G(X0), orange 85B, and yellow Y(K2) are examples.
Didymium Filters
These are special filters to improve the reproduction of certain biological
stains (such as Eosin). It is a recommended filter to be used when looking
at histology sections with H&E stain. The filters SCHOTT BG-20 and HOYA V-10
are examples.
Color Correction Filters
These filters are used to change the color temperature of of the light
source illuminating an object. The glass filters from HOYA type 80A and 80B
are examples.
Polarizing Filters
These filters have a preferred vibrational direction and hence transmit
preferentially light whose plane of polarization is parallel to a particular
axis of these filters. The filters from HOYA type PL and PL-CIR are
examples.
Blocking Filters
These filters block frequencies above or below a certain frequency. Examples
are UV and IR blocking filters. IR blocking filters are also known as heat
filters. The filter SCHOTT KG1 is an example.
Interference Filters
Instead of selective absorption and transmission, these filters function by
selective reflection and transmission. The have the advantage of providing a
narrow bandwidth and usually avoid the built-up of heat. Green interference
filters are an example, which are used for phase contrast microscopy.
Neutral Density or Grey Filters
These filters are used to decrease the intensity of light but keep the color
temperature unchanged. The glass filters from HOYA type NDx2, NDx4, and NDx8
are examples.
>I have a microscope which came with a blue and a green filter. I use the
>blue to
> create a white background. I can find anyone that knows what you would use
> the
> green filter for. Any help?