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Natural Science Forum / Physics / Optics / June 2005Lense swap
Hello, has anyone experience in swapping lenses of different companies between scopes? we currently have a Nikon Eclipse TS100 of which the image quality isn't smashing; all lenses have low N.A.. I am currently looking into some Olympus lenses which would improve our scope a great deal. Question is whether this works... Thanx in advance! Cheers Mathein What type of Olympus lenses would work on a CFI60 system?! The infinity systems of Olympus and Nikon are sufficiently different. Buy the CFI60 high-end lenses for inverted scopes from Nikon instead (long WD and coverglass correction of 1.2mm). BTW, the TS100 is a lower cost routine frame (similar to the E200 in quality). If one needs a higher optical performance, it would be best also to upgrade the frame and purchase an entirely new scope. APOs or FLUORs on a TS100 aren't a too good investment. Gregor > Hello, > has anyone experience in swapping lenses of different companies between [quoted text clipped - 3 lines] > deal. Question is whether this works... Thanx in advance! > Cheers Mathein I don't know yet which olympus lenses would work, I just know they're great on a olympus scope. I agree on the new aqcuisition however, as usual, resources are limited. The TS100 is in the house and it will be either that one or non. I intend to use coverglass with a thickness of ~ 0.15 mm on which living cells will grow. I got a quote from Nikon for some new lenses; a.o. CFI Plan Fluor DLL-10 x. Is that from the CFI60 line? This one will fit my demands most however hasn't got phase optics and only 0.3 N.A.. And costs a lot... Mathein Do you put/grow your specimen on top of a coverglass at 0.17mm? Usually an inverted microscope is used in such a way that the dish or slide is towards the lens. So the first glass surface after the front lens of the objective has a thickness of around 1.2mm for an inverted scope. BTW, the DLL is a phase lens for regular frames (not inverted once). The cost of roughly $699 is rather close to the Plan Fluor 40x ($876), which offers an NA of 0.75. But I think that you should take one or two of the following phase lenses if you are indeed using 1.2mm for coverglass correction: CFI Plan Fluor DL 10x (not DLL!), which has CG 1.2mm and Ph1. CFI Plan Fluor ELWD ADL 40x (NA 0.60), which has a correction collar that works for 0-2mm Ph2. CFI Plan Fluor ELWD DM 40x (NA 0.60), which has a correction collar that works for 0-2mm Ph2. ADL works great if your sample size is appropriate. Otherwise use DM or DL. BTW, I assumed that you have a phase condenser on your scope. Gregor PS: For more on ADL, DL, DLL, and DM look at http://www.microscopyu.com/articles/phasecontrast/phaseconfiguration.html >I don't know yet which olympus lenses would work, I just know they're > great on a olympus scope. I agree on the new aqcuisition however, as [quoted text clipped - 6 lines] > > Mathein Just a stupid question. Does your TS100 come with lenses that are corrected for 1.2mm coverglass thickness and are you using them on glass surfaces much thinner than 1.2mm? If yes, no wonder the optical performance is very poor. Gregor > Do you put/grow your specimen on top of a coverglass at 0.17mm? Usually an > inverted microscope is used in such a way that the dish or slide is [quoted text clipped - 29 lines] >> >> Mathein Indeed I grow the cells on top of the 0.17 mm glass (this all resides in a chamber containing medium to keep the cells happy). Come to think of it, I guess you're right; here are the specifications of the lenses: Nikon 10x/0.25 N.A. Ph1 ADL Infinity/1.2 WD 6.2 Nikon 40x/0.55 N.A. Ph1 ADL Infinity/1.2 WD 2.1 I did get the Nikon sales rep in however he didn't know a lot. He sent me a quote for an update which were: CFI Plan FLuor DLL-10x/0.30 N.A. Ph1, WD 16 CFI Plan FLuor DLL-40x/0.75 N.A. Ph1, WD 0.72 Both were way above 1000 euros per piece... I also still have to buy a camera and acquisition software so every penny counts. Besides, the knowledge of the Nikon guy didn't give me too much confidence in their business. Nonetheless, I'll try to arrange some testing period. Mathein This is a typical Nikon sales rep response. Instead of telling his customer why the performance is poor, just issued a quote for expensive lenses. In this case, try to get a cheap Nikon CFI60 achromat (with or without phase). (Your sales rep shall get you one.) You should see a great improvement. If satisfied, ask your sales rep to quote you for the CFI60 Achromate phase lenses. If you do not require the extra long working distance (ELWD) versions, you have the choice between Plan Achro Phase and Achro Phase. Quite often the Achro Phase lenses are enough. But get the right phase condenser. How much did he quote you on the phase condenser? Is it the one with a turret? Gregor > Do you put/grow your specimen on top of a coverglass at 0.17mm? Usually an > inverted microscope is used in such a way that the dish or slide is towards > the lens. So the first glass surface after the front lens of the objective > has a thickness of around 1.2mm for an inverted scope. BTW, the DLL is a <snip> A colleague introduced me to these awesome petri dishes that have a #1.5 coverslip in the center, like a window. So, one can grow adherent cells normally, and then use the dish on an inverted scope with the proper coverslip thickness already in place. I forget who makes them.... but they are not too expensive.  SignatureAndrew Resnick, Ph.D. Department of Physiology and Biophysics Case Western Reserve University >> Do you put/grow your specimen on top of a coverglass at 0.17mm? >> Usually an inverted microscope is used in such a way that the dish or [quoted text clipped - 9 lines] > coverslip thickness already in place. I forget who makes them.... but > they are not too expensive. Possibly LabTek? http://www.nuncbrand.com/page/en/235.aspx Cheers Franco >> A colleague introduced me to these awesome petri dishes that have a >> #1.5 coverslip in the center, like a window. So, one can grow [quoted text clipped - 5 lines] > > http://www.nuncbrand.com/page/en/235.aspx Close- some people here use those also. Nope, I was thinking of MatTek. http://www.glass-bottom-dishes.com/ SignatureAndrew Resnick, Ph.D. Department of Physiology and Biophysics Case Western Reserve University > Hello, > has anyone experience in swapping lenses of different companies between [quoted text clipped - 3 lines] > deal. Question is whether this works... Thanx in advance! > Cheers Mathein There are a bunch of problems but the main one is that CF160 and Olympus objectives have different screw threads. That causes a whole lot of problems! Next you have correction problems. The real problem is it sounds like the real issue here is using a long working distance system when you need the numerical aperature (NA) of a short working distance system. As some of the posters have suggested there are aproaches to this problem readily availible. You might want to rethink the instrumentation part of your experimental design. Kevin Cunningham SMS |
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