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Natural Science Forum / Physics / Optics / November 2005



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Cooled CCD cameras - Recommendations?

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hbarwood@troy.edu - 27 Nov 2005 15:36 GMT
I could use some input from any users of cooled CCD cameras regarding
types and prices. Is there a good, inexpensive camera out there with
decent resolution? I've visited a few "scientific" camera sites and
they, as is typical of scientific instruments, do not give you any idea
of pricing. I'm considering purchasing a low-light color camera, but
would like to be able to use it with external IR filters so I can also
image in the NIR. All suggestions welcome. Thanks.

Henry Barwood
hbawood@troy.edu
Gary G - 27 Nov 2005 17:10 GMT
>I could use some input from any users of cooled CCD cameras regarding
>types and prices. Is there a good, inexpensive camera out there with
[quoted text clipped - 6 lines]
>Henry Barwood
>hbawood@troy.edu

Why does it need to be cooled?  What do you consider to be decent
resolution--pixel count in this regard?

Gary Gaugler, Ph.D.
Microtechnics, Inc.
Granite Bay, CA 95746
916.791.8191
gary@microtechnics dot com
Gary G - 28 Nov 2005 00:22 GMT
>>I could use some input from any users of cooled CCD cameras regarding
>>types and prices. Is there a good, inexpensive camera out there with
[quoted text clipped - 9 lines]
>Why does it need to be cooled?  What do you consider to be decent
>resolution--pixel count in this regard?

I sell Pixera digital cameras and Optem microscope adapters.

150ES 1.5M pixel un-cooled C-mount camera with software  $4000 MSRP
$3600 plus adapter

150CL 1.5M pixel cooled (-20 below ambient) C-mount camera with
software  $5000 MSRP $4500 plus adapter

600ES 5.8M pixel un-cooled C-mount camera with software  $6000 MSRP
$5400 plus adapter

600CL 5.8M pixel cooled (-20 below ambient) C-mount camera with
software  $7000 MSRP  $6300 plus adapter

These include live image capture preview at about 30fps, depending on
CPU speed and memory.  Plus, Studio which allows for simple editing of
the captured image.  The camera uses a dedicated PCI card for PC or
Mac (no PCI-X or compact PCI).  System includes camera, cable,
software and appropriate adapter (extra cost).  One year warranty
applies.  With qualified PO, 30 day no hassle return policy is
granted. If you don't like it, and don't mess the camera up, send it
all back for full refund or cancellation of your PO.

Occasionaly I have a demo unit that is priced about 50% off of MSRP.
Currently I do not have any of these that are not already spoken for.

Microscope adapters are typically $395 for normal units or $1300 for
high resolution units.  These are standard from Optem.  You can buy
them yourself or I can include them in the system package.  Standard
units are typically in stock.  The high rez units are about two weeks
out.

These cameras have .01Lux sensitivity and use zero defect CCD imagers.
See my Web page or http://www.pixera.com for technical details.

The un-cooled cameras will take excellent pix with just ambient light.
No kidding.  Turn off the microscope lamp and do a 45 second exposure.
Perfect.  Turn on the lamp and get a perfect exposure in 1/100th
second.  So the point is that for most applications, cooled is not
needed.  The un-cooled cameras are so good that they work great as-is.

Very cool (no pun intended).

Gary Gaugler, Ph.D.
Microtechnics, Inc.
Granite Bay, CA 95746
916.791.8191
gary@microtechnics dot com
GTO - 28 Nov 2005 03:08 GMT
> 150CL 1.5M pixel cooled (-20 below ambient) C-mount camera with
> software  $5000 MSRP $4500 plus adapter

Very nice. Prices are coming slowly into the range of affordability ;-)

> These include live image capture preview at about 30fps, depending on
> CPU speed and memory.  Plus, Studio which allows for simple editing of

I thought it supports preview at 15fps @ 640x480. 30fps at full resolution
would be great.

Gregor
Gary G - 28 Nov 2005 04:09 GMT
>> 150CL 1.5M pixel cooled (-20 below ambient) C-mount camera with
>> software  $5000 MSRP $4500 plus adapter
[quoted text clipped - 8 lines]
>
>Gregor

If your cpu is fast enough, you can get up to 30fps or more live
preview.  This also applies to the active focus bars.  On a PC, 913MHz
works fine...more than this does even better.  I run 913MHz and 2.8GHz
and am happy with both scenarios.  The point is that less than 913MHz
is not going to be really satisfactory.

Live preview is at native CCD resolution. So for 150-series, what you
see is what you get.  For 600-series, preview is at 150 resolution so
that the diractor does not come into play.  In the end, it makes no
difference.  Either you get a nice 1.5M pixel image or a 5.8M pixel
image.  With the 600-series, you can opt for a single shot 1.5m pixel
image or a multi-shot Diractor 5.8M pixel image.

Gary Gaugler, Ph.D.
Microtechnics, Inc.
Granite Bay, CA 95746
916.791.8191
gary@microtechnics dot com
justbeats - 29 Nov 2005 08:27 GMT
> The un-cooled cameras will take excellent pix with just ambient light.

That's an interesting point...

What effect does "ambient" light have on resolution compared to (say)
Kohler illumination? Similar to darkfield or oblique illumination I
guess?

   Cheers
   Beats
Gary G - 30 Nov 2005 04:36 GMT
>> The un-cooled cameras will take excellent pix with just ambient light.
>
[quoted text clipped - 6 lines]
>    Cheers
>    Beats

The issue here is that the camera can make a "decent" image using only
room light rather than formal illumination.  It will auto color
correct.  With dry 40X and lower mag objectives, the results are good.
Certainly, using a condenser is better than ambient light.  The point
is/was to show how sensitive the camera is.  There is no suggestion
that using this camera would eliminate a condenser.

Gary Gaugler, Ph.D.
Microtechnics, Inc.
Granite Bay, CA 95746
916.791.8191
gary@microtechnics dot com
hbarwood@troy.edu - 28 Nov 2005 16:41 GMT
Hi Gary,

I was looking for something in the range of 3+ megapixels. I use
various webcams and a Nikon CP990 for photography, but all suffer from
thermal noise on long exposures.

What I'm doing is cathodoluminescence microscopy. With film, CL work
amounts to long exposures (minutes, sometimes). For digital work, I
take a series of exposures and sum them together. Unfortunately, noise
builds up using this method and really detracts from the images, both
aesthetically and scientifically.

I've modified an old 3Com webcam to work in the NIR and take
simultaneous images of the infrared luminescence of my samples. The
webcam has about 3 lux sensitivity, but is limited to 1/4 second
maximum exposure times. I would like to work with a higher sensitivity
and higher resolution camera than my webcam.

Perhaps this clarifies my question a bit. Thanks for helping out.

Henry
Kevin Cunningham - 28 Nov 2005 21:35 GMT
> Hi Gary,
>
[quoted text clipped - 17 lines]
>
> Henry

Henry, Take a look at the QImaging site(www.qimaging.com).  They offer at
least one IR camera.  So does Diagnostics (www.diaginc.com).  Gary's stuff
is usually very good.  When you talk to your local rep please be specific
about the application.  I can remember selling a person an image analyser in
about 30 min.  Then 2 weeks later I went up to Athens, GA to show him how it
worked.  It didn't work at all for him.  He really needed to tell me what he
wanted but that never happened.

Thanks,

Kevin Cunningham
SMS
Gary G - 29 Nov 2005 06:38 GMT
>Hi Gary,
>
[quoted text clipped - 17 lines]
>
>Henry

The Pixera digicams use 100% perfect CCDs, and when cooled, are
totally awesome.  Even the non-cooled units use 100% perfect CCDs.
The issue is whether you need cooled or not.  

I can take perfect pictures with ambient light on a slide....yep.  It
takes 20-50 seconds to do this.  But turn on the transmitted light
house and exposure drops to 1/100 second.  

With a cooled Pixera, you will not see noise.  However, your choices
are limited to 1.5M pixels or 5.8M pixels.  They do not offer any
other values.  But for most documentary work, the 1.5M pixel unit
works fine.  The sensitivity of these cameras is 0.01Lux.  Dynamic
range is 12 bits.

With the cooled Pixera, you need only take one shot.  Depending on
what your final output is, you may only need a 1.5M pixel unit.

Gary Gaugler, Ph.D.
Microtechnics, Inc.
Granite Bay, CA 95746
916.791.8191
gary@microtechnics dot com
Chuck Olson - 27 Nov 2005 18:56 GMT
Try the sci.astro.amateur newsgroup.

> I could use some input from any users of cooled CCD cameras regarding
> types and prices. Is there a good, inexpensive camera out there with
[quoted text clipped - 6 lines]
> Henry Barwood
> hbawood@troy.edu
GTO - 27 Nov 2005 20:17 GMT
Look at the ST-2000XM and ST-2000XMI from SBIG (www.sbig.com). The 'I'
stands for the standard C-mount camera. These are Peltier cooled 1600x1200
CCD based image devices (monochrome) for which you need a color-filter-wheel
if you want to do color imaging. They use a 16-bit A/D (not just 14-bit) and
are very very sensitive. The best for weak fluorescence microscopy (e.g.
when used to monitor fluorescence from MALDI spots).

Disadvantages: The SW is geared towards Astronomy, but they provide a decent
API for own camera integration (which I once used). And, they are rather
slow due to their 16-bit A/D. So speed is just around one fps at full
resolution. Last but not least, the pixel size is 7.4um, which makes them
great for low S/N and not too great for direct projection of the
intermediate image. You will need the appropriate adapter when using them
with a Nikon Plan APO 4x NA 0.20, for example.

In 2003, I paid around US$3000 if I remember correctly. I have not yet found
a decent replacement for this camera that sells at a comparable price and is
made especially for microscopy and ships with appropriate SW. - Astronomers
are so lucky!

Gregor

>I could use some input from any users of cooled CCD cameras regarding
> types and prices. Is there a good, inexpensive camera out there with
[quoted text clipped - 6 lines]
> Henry Barwood
> hbawood@troy.edu
justbeats - 29 Nov 2005 08:21 GMT
> Astronomers are so lucky!

Hmmm, after freezing my buns off and struggling to get a stable image
through miles of wobbly atmosphere, I'm not so sure I agree :-) There's
a lot to be said for a working distances of microns rather than light
years...

Back to the topic - Starlight Express now have a large format version
of their colour CCD too (uses a Bayer filter). Might do the job better.
Saves multiple exposures through filters which is a big benefit.
However, for the specific application the OP stated, a monochrome will
probably do...

   Cheers
   Beats
 
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