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Natural Science Forum / Physics / Optics / December 2005



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Can anybody answer such a simple question?

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Eniac - 27 Dec 2005 14:32 GMT
how strong microscope should i have i want to se spermatozoids (sperms)?

thx

one sperm is 0,0005 mm...
Kevin Cunningham - 27 Dec 2005 22:07 GMT
> how strong microscope should i have i want to se spermatozoids (sperms)?
>
> thx
>
> one sperm is 0,0005 mm...

A microscope is neither strong nor weak, its a microscope.  Usually techs
working on human spermatazoa use a .65 NA or greater objective such as  a
40X plan achromat or a 100X objective.  You may find phase contrast or
Differential Interference Contrast to be especially valuable unless you
stain the sperm.

Kevin Cunningham
SMS
Dr. Georg N.Nyman - 28 Dec 2005 16:01 GMT
> how strong microscope should i have i want to se spermatozoids (sperms)?
>
> thx
>
> one sperm is 0,0005 mm...

As it was correctly stated already, 40x Plan Phase or DIC objective with a
10x eyepiece is plenty enough for this easy task. And you got the
dimensions wrong....sperms of the size you mentioned do not really exist
for humans (at least not the ones which are mature...) -or do you study
sperms of microorganisms :-))
rgds George Nyman
Aaron - 28 Dec 2005 23:43 GMT
This person is a gnome intent on baiting the unwary.  This same
question is posted here periodically by this person.

>how strong microscope should i have i want to se spermatozoids (sperms)?
>
>thx
>
>one sperm is 0,0005 mm...
Eniac - 29 Dec 2005 08:21 GMT
> This person is a gnome intent on baiting the unwary.  This same
> question is posted here periodically by this person.
[quoted text clipped - 4 lines]
>>
>>one sperm is 0,0005 mm...

Really I am not sure about what kind of problem you have.
1. This is a first (and last) time i posted this question. I wouldnt do that
anymore, cause i got an answer.
2. Can you explain your comment in detail? Periodically? What do you mean by
"periodically"?

regards
 
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