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Natural Science Forum / Physics / Optics / December 2005Axiocam images
The search for a cheap bypass to high-end dedicated microscope cameras is understanable, however, people might appreciate seeing these images. Here are four routine brightfield images taken with a Zeiss Axiocam HRc. The original files are too large for the Web. Although quite a bit is lost in compression, the images are suggestive of the capabilities of this camera. Two slides are human tissue samples and two are botanicals. The objective used was a Zeiss 10X 0.33NA 160mm planapo. Try blowing up the linden cross section to look at the small cellular details. This portion of the specimen is near the outer layers. The kidney section is stained with Mason's trichrome. http://home.comcast.net/~nghy/mywebpages/test/kidney.jpg http://home.comcast.net/~nghy/mywebpages/test/linden_cs.jpg http://home.comcast.net/~nghy/mywebpages/test/lung.jpg http://home.comcast.net/~nghy/mywebpages/test/ranunculus_cs.jpg Aaron >The search for a cheap bypass to high-end dedicated microscope cameras >is understanable, however, people might appreciate seeing these [quoted text clipped - 17 lines] > >Aaron Aaron did a great service to the group with these pix. If you compare these native images to Lucis processed images at http://www.microtechnics.com/kidneyl169.jpg http://www.microtechnics.com/linden_csl157.jpg http://www.microtechnics.com/lungl133.jpg http://www.microtechnics.com/ranunculus_csl135.jpg you will see a marked improvement in contrast and definition. Keep in mind that he did these pix with a 10X objective. Imagine the difference with a 40X or 60X or 100X objective. The point is that the captured image is not always the final one. The lxxx values are the big cursor values used with Lucis Pro. Each image needs a different cursor value depending on the overall tonal range, and what is actually being the subject of the picture. Gary Gaugler, Ph.D. Microtechnics, Inc. Granite Bay, CA 95746 916.791.8191 gary@microtechnics dot com Hi Gary, I chose slides packed with small details coupled with a lower powered objective to point out the ability of the Zeiss Axiocam to obtain details beyond most people's vision. The image of the Linden CS are expecially suited to make this point given the large amounts of small vascular structures in the specimen. I also avioded post proccessing to make the images prettier. Doubtless there is far more information recorded in a digital image that is first noticed. Lucis did an interesting job on the images. This type of post processing of digital images is nye on impossible with film photography. There is a program called Photoflair which has a routine called Retinex. Although Photoflair was designed for general photography use, the Retinex routine has been especially useful for police type forensic work. Retinex senses dark areas and brings out hidden details at the same time it senses bright highlights and brings out hidden details. The final processed image provides much more information especially from poor original images. . . BTW, Zeiss has a proprietery file format (.ZVI) that records the fundamental camera data plus complete database text and annotations. This format is very effient in saving storage space. The Axiovision software can then use the basic information to create clone images in numerous other formats. In this way the original data is unchanged and can be referenced many times. Nikon has a similar concept in the Nikon Electronic File (.NEF). I think both these trends should be of great interest to scientists. I only wish the different manufacturers would get together and set universal standards. Aaron >>The search for a cheap bypass to high-end dedicated microscope cameras >>is understanable, however, people might appreciate seeing these [quoted text clipped - 40 lines] >916.791.8191 >gary@microtechnics dot com >Hi Gary, > [quoted text clipped - 4 lines] >vascular structures in the specimen. I also avioded post proccessing >to make the images prettier. There is a crossover point somewhere where up to xx mag, a 1.5M pixel will do the job. But a 6M pixel camera will work better. Then, as mag increases, fewer pixels are needed. >Doubtless there is far more information recorded in a digital image >that is first noticed. Lucis did an interesting job on the images. >This type of post processing of digital images is nye on impossible >with film photography. Most all (~99%) of my post processing is done on native digital images. However, if the neg or slide is scanned correctly, Lucis works on it too. >There is a program called Photoflair which has a routine called >Retinex. Although Photoflair was designed for general photography [quoted text clipped - 3 lines] >details. The final processed image provides much more information >especially from poor original images. . . Photoflair sounds very much like Lucis. Lucis has been used for photography, forensics and old photo restoration. I did not see a writeup about what algorithm is used. At its price point, Photoflair may be worth a tryout. It, like Lucis and LucisArt have free demo periods. The other similar product is analySIS from Soft Imaging. Their package includes what they call DCE (Differential Contrast Enhancement) filter. It has many ajustment options. Too many, IMO. However, mostly I use analySIS for doing Z stacks for large DOF at high mag. Gary Gaugler, Ph.D. Microtechnics, Inc. Granite Bay, CA 95746 916.791.8191 gary@microtechnics dot com |
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