Infrared microscopy with a Nikon TE-2000U

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thx1138 - 23 Aug 2006 19:19 GMT

Hello,

I am about to put together a supercontinuum light source (coherent
white light 450-1600nm) and was considering trying to route the
infrared portion of the beam into my Nikon TE-2000U and try some
epi-fluorescent/scattering experiments.

I am concerned about absorption and attenuation from the
mirrors/objective in this wavelength range. I've asked Nikon, but no
one seems to know. Does anyone have any experience with this? Or should
I just use my typical course of action and try it and see what happens?

Any suggestions would be great.
Joel

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Kevin Cunningham - 26 Aug 2006 19:05 GMT

You might run into several problems. One is focus change, As you go up in
the ir range the focus will change. Then there's transmission, most glass
objectives will transmit only in the visible range cutting of the high ir.
You might want to look at catadioptic objectives.

Take a look at Nicolet-Thermo web
site:(www.thermo.com/com/cda/product/detail/1,,115490,00.html)

Of course remember, you can't see infrared so you will need an infrared
camera to see what's there. These have gotten less expensive over the
years.

Good luck, let us know how it goes!

Kevin Cunningham
SMS

> Hello,
>
[quoted text clipped - 10 lines]
> Any suggestions would be great.
> Joel

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Natural Science Forum / Physics / Optics / August 2006

Infrared microscopy with a Nikon TE-2000U