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Natural Science Forum / Physics / Optics / June 2005



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Lense swap

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Mathein - 21 Jun 2005 17:11 GMT
Hello,
has anyone experience in swapping lenses of different companies between
scopes? we currently have a Nikon Eclipse TS100 of which the image
quality isn't smashing; all lenses have low N.A.. I am currently
looking into some Olympus lenses which would improve our scope a great
deal. Question is whether this works... Thanx in advance!
Cheers Mathein
Bob Knowlden - 22 Jun 2005 00:47 GMT
The Eclipse TS100 appears to use CFI60 objectives. I believe that they are
proprietary to Nikon. I don't recall the mounting thread specification for
CFI60 objectives, but it's different from the traditional RMS thread.

Here's some Nikon propaganda:

http://www.microscopyu.com/articles/optics/nikoncfioptics.html

HTH.

Bob Knowlden

Address may be scrambled. Replace nkbob with bobkn.

> Hello,
> has anyone experience in swapping lenses of different companies between
[quoted text clipped - 3 lines]
> deal. Question is whether this works... Thanx in advance!
> Cheers Mathein
Johannes Swartling - 22 Jun 2005 09:43 GMT
> has anyone experience in swapping lenses of different companies between
> scopes? we currently have a Nikon Eclipse TS100 of which the image
> quality isn't smashing; all lenses have low N.A.. I am currently
> looking into some Olympus lenses which would improve our scope a great
> deal. Question is whether this works... Thanx in advance!

A couple of issues:

1. Different mounting threads. New Nikon scopes use M25, as do Leica.
Olympus and Zeiss use RMS. You can use adapter rings to mount RMS objectives
on M25 turrets, but not the other way around. Bottom line: you can mount
Olympus objectives on a Nikon frame with an adapter. I know Leica sells
adapter rings because I once bought one from them (at about £40).

2. Parfocal distance. May or may not be important to you.

2. Make sure to use infinity corrected objectives if it is a frame intended
for that.

3. Different focal length of the tube lens for inifinity corrected optics.
Nikon: 200 mm. Olympus: 180 mm. Will cause slightly different magnification
from what is written on the objective.

4. All achromatic correction done in objective vs. some done in the tube
lens. If I remember correctly, Nikon and Olympus objectives have all
correction in the objective, while Leica and Zeiss don't. So Nikon and
Olympus should be interchangable without loss of quality.

I've used a 50x, NA=0.50 LWD Olympus objective on a Nikon frame once, and it
worked nicely, but I didn't have an Olympus frame to compare it with and I
didn't do any quantitative evaluation of the resolution.

Johannes
Mathein - 28 Jun 2005 18:56 GMT
Thanks all for the useful thoughts, I've a guy from Olympus coming in
later this week so we'll see. Parfocal distance is not an issue, idea
is to acquire images at set intervals with the same lense i.e. I mostly
will use one lense. I'll keep the focal length in mind and calibrate
properly. I'll let you know how things work out!

Mathein
Andy Resnick - 22 Jun 2005 14:15 GMT
<crossposted to sci.tech.microscopy>

> Hello,
> has anyone experience in swapping lenses of different companies between
[quoted text clipped - 3 lines]
> deal. Question is whether this works... Thanx in advance!
> Cheers Mathein

As others point out, the answer is generally 'you can't".  I've used
Nikon objectives in a Zeiss scope, but that's the exception rather than
the rule.  A much better plan would be to understand why you are getting
(presumably) poor performance from your Nikon lenses.  If you provide us
with some specifics (lenses, cameras, etc..) that would be helpful-
maybe your microscope is simply misaligned: have you checked to ensure
either Kohler or critical illumination is present?  What objects are you
imaging to come to the conclusion that the image quality "isn't smashing"?

Another question is why aren't you simply looking at getting
higher-performance Nikon objectives?

Signature

Andrew Resnick, Ph.D.
Department of Physiology and Biophysics
Case Western Reserve University

Mathein - 28 Jun 2005 19:24 GMT
I guess the main problem is the low N.A.; I checked illumination and
they seem to be well aligned. I used a 40xPH2/0.75 NA universal Plan
fluorite WD 0.51 mm Olympus lense (on a IX81 Olympus scope) before and
the image was clearly brisker and sharper. I hope this all isn't caused
by the difference in body but merely a difference in lenses. Otherwise
it's gonna be expensive... I have looked into higher-performance lenses
(see above). I'll try and arrange a direct comnparison between both
Nikon and Olyumpus lenses and let you know.

Mathein
 
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