Natural Science Forum / Biology / Microbiology / May 2006

Dear Sir
 My question is
 1.  how to do electrphoresis and zymogram (activity satining of enzymes on electrophoretic gels) of extracellular enzymes from obligate halophiles since high NaCl possess problems in electrophoretic mobility.
 2. How to purify extracellular hydrolytic ...

Have heared that one may mix Pfu and Taq polymerases (Fermantas). Taq
is copying Pfu verifying. Have any of u tried this combbination? I'm
planning to introduce two nucleotide changes in 1kb DNA fragment. So
far I made set of 6 PCRs (I'm using so called SOE-PCR method with

im studying immobilization of aspergillus spores on ca-alginate beats.
i m using distiled water (containing C and N sources) for growth and I
change the media daily. but after 4 days, alginate beats deformed and
i didnt use it. what can i do for deformation.

Cold viruses infect the cells of the walls of cappilaries supplying the
hair cells in the organ of corti of the left cochlea. The viruses do
not kill those cells but transform them into a cancerous state.
Symptoms are:

A. Measles (encephalitis) virus infects cells of walls of cappilaries
supplying the brain. The virus does not kill those cells but irritates
them to cancer by tickling and itching them!
The symptoms are:

I need above mentioned information.
Can anyone point me to a lab that could help me makeing a genetically
modified bacterium and assist me in getting approval for it?
Best Regards,

It may seem like I am asking the same questions over and over again. I
am not. Please bear with me.
Can an anaerobic bacterium metabolize a saturated fatty acid in
anaerobic environment w/out *any* oxidation?

I'm trying to figure out what kind of cyanobacteria or algae would fit
the below requirements:
- uses photosynthesis for growing (does not eat anything but minerals
basically)

                                             EPSF Summer University
                                               10Th -24Th of July,
2006
                                                      Cairo, Egypt

If an anaerobic bacterium is made to feed only the saturated fatty
acids present in butter made from cow's milk, what compounds will the
bacterium excrete in its waste products directly as a result of
processing those fatty acids? Will those compounds smell bad?

I am writing a paper about environmental microbiology ecology. Could
somebody offer some available journals for these. Thanks a lort.

Why can't anaerobic bacteria metabolize saturated fatty acids in
anaerobic conditions? They can metabolize amino acids, so why can't
they metabolize saturated fatty acids?
Thanks,

Leaving aside the localised hotspots within milk heated by microwave, is
there any clear evidence against microwave heated babys milk?
TIA
Nick

Hello to you all,
I am searching for bacteria with special properties, and I wonder if
there is a database or at least a table where some basic
characteristics of many bacteria are collected.

Can anyone suggest me some websites where i can get recent journals on
Wastewater treatment which is needed for my research project.
thanks