Natural Science Forum / Biology / Microbiology / August 2006

Hi, I am looking for the same kind of database. did you find any?
Thanks
Anis

I am a graduate student, I plan to grow Trichoderma reesei (ATCC 26921)
and clone a gene inside it.
I could buy this srain from ATCC, however, I couldn't access to the old
publications describing how to grow it well and RNA extraction.

My son is doing a science project.  we will be taking swabs of certain
surfaces to see what is growing on them.
what shoud we use and where can we buy these materials.
help !

Hai,
 does anyone know how to make crude Staphylococcal beta toxin, for identification of Streptococcus agalactiae from primery culture, is there any other effective method for identification for Streptococcus agalactiae beside Camp test and Lancefield's serrogrouping test  

If not, can we get one?  There has to be some way of moderating what is
posted on this board.
--Alex

I was recently surprised to find out that in aerobic conditions +
glucose medium E. Coli almost doesn't rely on the TCA cycle to produce
energy i.e. all of the energy is produced from glycolysis (+respiration
used the NADH glycolysis produces I guess), at least it is so in the

Does anybody know how we can enumerate non-cultureable but viable bacteria (E.Coli) in the sea water
to check environmental contamination? your answer will be really useful for me and I appreciate it.
Reza

Penicillin:
    A Paradigm for Biotechnology
Richard I. Mateles, Editor
Reprints The History of Penicillin Production, the classic publication of

hai
i just want to know what's the formula for making methylene blue milk for detecting Streptococcus Group D and how to determine positive and negative reaction
thanks
---------------------------------

Hi people. I hope I can get a few sources where I can understand the
factor that determine the host range of a viral infection.
I know the factors are :
- susceptibility

What is microbial loading? When and where should it be done? Why is it
that one has to choose plates that contain only 30 - 300 colonies?
I'm doing an experiment that uses samples from the lake and pond and
culturing the microorganisms in it using the streak and pour plate

I want to count Lactobacilli in selfmade yoghurt.
Should be easy, cheap and give an accuracy of about half a decade
(favtor 3 uncertainity).
Can I just homogenize a sample of the yoghurt, add DAPI, incubate it 15

What chemicals other than diacetyl, butryic acid, isobutryic acid,
propionic acid, valeric acid, and isovaleric acid are responsible for
the cheesy foot odor?
I am confused about methane thiol it MAYbe also resposible for foot

Has anybody tried to renature proteins bound to Ni-NTA (nickel-agarose)
column? I use Qiagen protocol (washing the resign by linear gradent of
urea in pH=8,0 buffer from 6M to 0,75M). However protein is not folded
(CD,HPLC) moreover it dissapeared stored in 20% glycerol buffer (used

A long long time ago the forefront of science used to be in the garden
sheds of eccentric victorian upper class and thus it was relatively
easy to make discoveries worth writing about and worth publishing.
These days its so much harder, most stuff that can be done in a shed